Year : 2018 | Volume
: 17 | Issue : 1 | Page : 5--8
Role of aberrant glycosylation enzymes in oral cancer progression
Bhairavi N Vajaria, Kinjal A Patel, Prabhudas S Patel
Department of Cancer Biology, Molecular Oncology Laboratory, The Gujarat Cancer and Research Institute, Ahmedabad, Gujarat, India
BACKGROUND: Carcinogenesis, a multistep process involves sequential changes during neoplastic transformation. The various hallmarks of cancer aid in cell survival, proliferation, and dissemination. Aberrant glycosylation, a recently defined hallmark of cancer, is influenced by glycosylation enzymes during carcinogenesis. Therefore, the present study measured α-2,3 and α-2,6 sialyltransferase (ST), sialidase, and α-L-fucosidase activity in patients with oral precancerous conditions (OPC) and oral cancer patients.
SUBJECTS: The study enrolled 100 oral cancer patients, 50 patients with OPC, 100 healthy controls, and 46 posttreatment follow-ups of oral cancer patients. Blood and saliva were collected from all the participants.
MATERIALS AND METHODS: Sialidase activity was measured by spectrofluorimetric method, α-2,3 and α-2,6 ST by ELISA using biotinylated lectins, and α-L-fucosidase by spectrophotometric method.
RESULTS: The results depicted increased levels of sialidase, α-2,3 and α-2,6 ST, α-L-fucosidase in patients with OPC and oral cancer patients. Receiver operating characteristic curve indicated significant discriminatory efficacy in distinguishing controls and oral cancer patients for serum and salivary sialidase and α-L-fucosidase activity, and serum α-2,6 ST. Furthermore, serum and salivary α-L-fucosidase activity and serum sialidase activity significantly distinguished controls and patients with OPC. Serum and salivary sialidase, α-L-fucosidase, and serum α-2,3 ST activity were higher in patients with metastasis as compared to nonmetastatic patients. Higher values of serum α-L-fucosidase activity were significantly associated with low-overall survival.
CONCLUSION: The increased levels of enzymes correlated with tumor initiation, progression, and metastasis in oral cancer patients. The alterations in glycosyltransferases/glycosidases thus support the view of glycosylation as a hallmark of cancer.
Prabhudas S Patel
Department of Cancer Biology, The Gujarat Cancer and Research Institute, Asarwa, Ahmedabad - 380 016, Gujarat
|How to cite this article:|
Vajaria BN, Patel KA, Patel PS. Role of aberrant glycosylation enzymes in oral cancer progression.J Carcinog 2018;17:5-8
|How to cite this URL:|
Vajaria BN, Patel KA, Patel PS. Role of aberrant glycosylation enzymes in oral cancer progression. J Carcinog [serial online] 2018 [cited 2019 Apr 23 ];17:5-8
Available from: http://www.carcinogenesis.com/article.asp?issn=1477-3163;year=2018;volume=17;issue=1;spage=5;epage=8;aulast=Vajaria;type=0