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  Indian J Med Microbiol
 

Figure 1: KCNQ1 gene targeting in golden Syrian hamsters by CRISPR/Cas9. (a) Schematic diagram for the hamster KCNQ1 genomic locus and the site targeted by the sgRNA. Letters in red are the PAM sequence, letters in green are the sgRNA targeting sequence, and the restriction enzyme recognition site (BglI) used for the polymerase chain reaction-restriction fragment length polymorphism assay is underlined. (b) Primer sequences for genotyping. (c) Gel images from the baby hamster kidney cell transfection assay results indicating the success of the design and genotyping and efficiency before pronuclear injection. Left, uncut with BglI; right, cut with BglI

Figure 1: <i>KCNQ1 gene targeting in golden Syrian hamsters by CRISPR/Cas9</i>. (a) Schematic diagram for the hamster <i>KCNQ1</i> genomic locus and the site targeted by the sgRNA. Letters in red are the PAM sequence, letters in green are the sgRNA targeting sequence, and the restriction enzyme recognition site (BglI) used for the polymerase chain reaction-restriction fragment length polymorphism assay is underlined. (b) Primer sequences for genotyping. (c) Gel images from the baby hamster kidney cell transfection assay results indicating the success of the design and genotyping and efficiency before pronuclear injection. Left, uncut with BglI; right, cut with BglI