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  Indian J Med Microbiol
 

Figure 2: Establishment of a breeding colony of KCNQ1 knockout hamsters. (a) Indels introduced into KCNQ1 in 3 F0 founder hamsters. wild-type; Δnt and + nt: nucleotide deletions and insertions, respectively. The +11nt allele in F0F7 is the result from the replacement of a CT dinucleotides by 13 random nucleotides. (b) Genotyping result of the PCR-RFLP assay on a litter of F0 founder hamsters. F0F6 carries a Δ 9nt indel and F0F7 carries both +1nt and +11nt indels. (c) Genotyping results with the PCR-RFLP assay on F1 pups produced from breeding an F0F7 (female) with a WT male. Indels in each of the targeted pups were revealed by Sanger sequencing. (d) Genotyping results by the polymerase chain reaction-restriction fragment length polymorphism assay on F2 pups produced from sister-brother breeding between two heterozygous F1 pups (#6 and #8) both carry a +11nt allele from the F0F7 founder as indicated in C)

Figure 2: Establishment of a breeding colony of KCNQ1 knockout hamsters. (a) Indels introduced into <i>KCNQ1</i> in 3 F0 founder hamsters. wild-type; Δnt and + nt: nucleotide deletions and insertions, respectively. The +11nt allele in F0F7 is the result from the replacement of a CT dinucleotides by 13 random nucleotides. (b) Genotyping result of the PCR-RFLP assay on a litter of F0 founder hamsters. F0F6 carries a Δ 9nt indel and F0F7 carries both +1nt and +11nt indels. (c) Genotyping results with the PCR-RFLP assay on F1 pups produced from breeding an F0F7 (female) with a WT male. Indels in each of the targeted pups were revealed by Sanger sequencing. (d) Genotyping results by the polymerase chain reaction-restriction fragment length polymorphism assay on F2 pups produced from sister-brother breeding between two heterozygous F1 pups (#6 and #8) both carry a +11nt allele from the F0F7 founder as indicated in C)