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  Indian J Med Microbiol
 

Figure 2: Gel images of optimization conditions for LAMP assay. (a) Temperature optimization: Lanes marked as 55, 60, 62, 65, and 70 indicate thedifferent incubation temperatures (°C), NC: Negative control. (b) Time optimization: Lanes marked as 30, 45, 60, and 75 indicate the incubation time (in min), NC: Negative control. (c) Specificity of LAMP assay: Results of LAMP assay on addition of four primers (L4), three primers (L3), two primers (L2), and one primer (L1), NC: Negative control. (d) Sensitivity of LAMP assay: 100-10-9 indicate the tenfold serial dilution with starting concentration of 100 ng (100) of DNA, NC: Negative control

Figure 2: Gel images of optimization conditions for LAMP assay. (a) Temperature optimization: Lanes marked as 55, 60, 62, 65, and 70 indicate thedifferent incubation temperatures (°C), NC: Negative control. (b) Time optimization: Lanes marked as 30, 45, 60, and 75 indicate the incubation time (in min), NC: Negative control. (c) Specificity of LAMP assay: Results of LAMP assay on addition of four primers (L4), three primers (L3), two primers (L2), and one primer (L1), NC: Negative control. (d) Sensitivity of LAMP assay: 10<sup>0</sup>-10<sup>-9</sup> indicate the tenfold serial dilution with starting concentration of 100 ng (10<sup>0</sup>) of DNA, NC: Negative control