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  Indian J Med Microbiol
 

Figure 4: A representative image of EGFR T790M mutation assay by ddPCR: A representative image (1D plot from QuantaLife software) of EGFR T790M mutation assay by ddPCR. Two probes, one labeled with FAM (for mutant) and another with HEX (wild type), were used. The blue dots indicate the presence of FAM-labeled droplets positive for mutant allele (D04, upper panel), whereas green dots indicate the HEX-labeled droplets positive for wild-type allele (D04, lower panel). The channel amplitudes are plotted in Y-axis separately FAM and HEX, whereas the events (number of droplets) are plotted in X-axis. The magenta line indicates the baseline and the black dots indicate droplets without any DNA

Figure 4: A representative image of EGFR T790M mutation assay by ddPCR: A representative image (1D plot from QuantaLife software) of EGFR T790M mutation assay by ddPCR. Two probes, one labeled with FAM (for mutant) and another with HEX (wild type), were used. The blue dots indicate the presence of FAM-labeled droplets positive for mutant allele (D04, upper panel), whereas green dots indicate the HEX-labeled droplets positive for wild-type allele (D04, lower panel). The channel amplitudes are plotted in Y-axis separately FAM and HEX, whereas the events (number of droplets) are plotted in X-axis. The magenta line indicates the baseline and the black dots indicate droplets without any DNA