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  Indian J Med Microbiol
 

Figure 2: Analysis of off-site target candidates at predicted sites in the golden Syrian hamster genome. (a) The top eight predicted off-site targets for mutagenesis. The mismatch sequence is labeled in red and protospacer adjacent motif is indicated in bold letters. (b) Polymerase chain reaction products flanking each predicted off-target site (#1-8) in wild type and mutant founder (F0#2) hamster. (c) Polymerase chain reaction product sequencing confirmed no off-target cleavage. A representative sequencing analysis of F0#2 is shown

Figure 2: Analysis of off-site target candidates at predicted sites in the golden Syrian hamster genome. (a) The top eight predicted off-site targets for mutagenesis. The mismatch sequence is labeled in red and protospacer adjacent motif is indicated in bold letters. (b) Polymerase chain reaction products flanking each predicted off-target site (#1-8) in wild type and mutant founder (F0#2) hamster. (c) Polymerase chain reaction product sequencing confirmed no off-target cleavage. A representative sequencing analysis of F0#2 is shown