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  Indian J Med Microbiol
 

Figure 2: Srcasm downregulates Fyn and FynY528F while c-Cbl preferentially downregulates Fyn Y528F. (a) COS cells were transfected with either Fyn or FynY528F at 0.5 μg and with increasing amounts of Srcasm (0.5, 1.0, and 1.5 μg). Cells were lysed after 16 h. Lysates were subjected to SDS-PAGE and analyzed by western blotting to detect Fyn, Srcasm, and β-actin. Fyn downregulation is inversely proportional to Srcasm levels, while FynY528 downregulation occurs in a dose-dependent manner. (b) COS cells were transfected with either Fyn or FynY528F at 0.5 μg and with increasing amounts of c-Cbl (0.5, 1.0, and 1.5 μg). Cells were lysed and lysates were subjected to SDS-PAGE and blotted for Fyn, c-Cbl, and β-actin. At all doses, c-Cbl effectively downregulates FynY528 while Fyn levels remain unchanged. Data representative of three experiments

Figure 2: Srcasm downregulates Fyn and FynY528F while c-Cbl preferentially downregulates Fyn Y528F. (a) COS cells were transfected with either Fyn or FynY528F at 0.5 μg and with increasing amounts of Srcasm (0.5, 1.0, and 1.5 μg). Cells were lysed after 16 h. Lysates were subjected to SDS-PAGE and analyzed by western blotting to detect Fyn, Srcasm, and β-actin. Fyn downregulation is inversely proportional to Srcasm levels, while FynY528 downregulation occurs in a dose-dependent manner. (b) COS cells were transfected with either Fyn or FynY528F at 0.5 μg and with increasing amounts of c-Cbl (0.5, 1.0, and 1.5 μg). Cells were lysed and lysates were subjected to SDS-PAGE and blotted for Fyn, c-Cbl, and β-actin. At all doses, c-Cbl effectively downregulates FynY528 while Fyn levels remain unchanged. Data representative of three experiments