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  Indian J Med Microbiol
 

Figure 3: Src Y527F mutant is downregulated by c-Cbl but not Srcasm. COS cells were transfected with either 0.5 μg (l) or 1.0 μg (h) of Src Y527F and either low or high doses of c-Cbl (2.0 and 2.5 μg) or Srcasm (1.0 and 1.5 μg). After a 16-h incubation, cells were lysed and lysates were subjected to SDS-PAGE and analyzed by western blot. At both low and high doses, c-Cbl downregulates Src Y-F while Srcasm does not. Data representative of three experiments. In this figure, the image of the lane for c-Cbl was cropped at both ends denoted by black rectangles

Figure 3: Src Y527F mutant is downregulated by c-Cbl but not Srcasm. COS cells were transfected with either 0.5 μg (l) or 1.0 μg (h) of Src Y527F and either low or high doses of c-Cbl (2.0 and 2.5 μg) or Srcasm (1.0 and 1.5 μg). After a 16-h incubation, cells were lysed and lysates were subjected to SDS-PAGE and analyzed by western blot. At both low and high doses, c-Cbl downregulates Src Y-F while Srcasm does not. Data representative of three experiments. In this figure, the image of the lane for c-Cbl was cropped at both ends denoted by black rectangles