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  Indian J Med Microbiol
 

Figure 4: Differential sensitivity of Srcasm-dependent and c-Cbl-dependent Fyn downregulation to lysosomal and proteosomal inhibitors. (a) COS cells were transfected with expression vectors containing Fyn and Srcasm indicated and incubated for 16 h. Cells were then incubated with lysosomal inhibitors, NH4Cl or Bafilomycin (Baf.) for 1 or 3 h. Some cells were incubated with a combination of proteosomal inhibitors (Prot.): lactacystin, MG132, and ALLN for 1 or 3 h. Cells were lysed using RIPA and analyzed by western blot. Data representative of two experiments. (b). COS cells were transfected with expression vectors containing Fyn Y528F and c-Cbl as indicated and incubated for 16 h. Cells were then treated as in A. Data representative of two experiments

Figure 4: Differential sensitivity of Srcasm-dependent and c-Cbl-dependent Fyn downregulation to lysosomal and proteosomal inhibitors. (a) COS cells were transfected with expression vectors containing Fyn and Srcasm indicated and incubated for 16 h. Cells were then incubated with lysosomal inhibitors, NH<sub>4</sub>Cl or Bafilomycin (Baf.) for 1 or 3 h. Some cells were incubated with a combination of proteosomal inhibitors (Prot.): lactacystin, MG132, and ALLN for 1 or 3 h. Cells were lysed using RIPA and analyzed by western blot. Data representative of two experiments. (b). COS cells were transfected with expression vectors containing Fyn Y528F and c-Cbl as indicated and incubated for 16 h. Cells were then treated as in A. Data representative of two experiments