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  Indian J Med Microbiol
 

Figure 1: Lentiviral-mediated transduction of the MD Anderson–metastatic breast cancer 231 cell line to overexpress angiopoietin-like protein 4-mGFP or mGFP. (a) Ectopic expression of angiopoietin-like protein 4 mGFP in the MD Anderson–metastatic breast cancer 231 cell line assessed by Western blot. (b) Secreted angiopoietin-like protein 4 from the MD Anderson–metastatic breast cancer 231 mGFP and MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 mGFP cell lines, respectively, was measured by ELISA. The mean concentration of angiopoietin-like protein 4 secreted from MD Anderson–metastatic breast cancer 231 mGFP and MD Anderson–metastatic breast cancer 231 Angiopoietin-like protein 4 mGFP measured 0.022 ng/ml and 0.87725 ng/ml, respectively. n = 3; P value was calculated with the Student's t-test using GraphPad Prism version 8 for Windows, GraphPad Software, La Jolla California USA, www.graphpad.com. The error bars indicate the standard deviation. (c) Micrographs of mGFP expressed by MD Anderson–metastatic breast cancer 231 mGFP or MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 –mGFP

Figure 1: Lentiviral-mediated transduction of the MD Anderson–metastatic breast cancer 231 cell line to overexpress angiopoietin-like protein 4-mGFP or mGFP. (a) Ectopic expression of angiopoietin-like protein 4 mGFP in the MD Anderson–metastatic breast cancer 231 cell line assessed by Western blot. (b) Secreted angiopoietin-like protein 4 from the MD Anderson–metastatic breast cancer 231 mGFP and MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 mGFP cell lines, respectively, was measured by ELISA. The mean concentration of angiopoietin-like protein 4 secreted from MD Anderson–metastatic breast cancer 231 mGFP and MD Anderson–metastatic breast cancer 231 Angiopoietin-like protein 4 mGFP measured 0.022 ng/ml and 0.87725 ng/ml, respectively. <i>n</i> = 3; <i>P</i> value was calculated with the Student's <i>t</i>-test using GraphPad Prism version 8 for Windows, GraphPad Software, La Jolla California USA, www.graphpad.com. The error bars indicate the standard deviation. (c) Micrographs of mGFP expressed by MD Anderson–metastatic breast cancer 231 mGFP or MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 –mGFP